ASCC1 Polyclonal antibody

ASCC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12301-1-AP

Synonyms

SMABF2, CGI18, CGI 18, ASCC 1, ASC1p50

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Validation Data Gallery

• 

  WB analysis using 12301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12301-1-AP (ASCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12301-1-AP targets ASCC1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASCC1 fusion protein Ag2946
• Full Name: activating signal cointegrator 1 complex subunit 1
• Calculated Molecular Weight: 45.5 kDa
• Observed Molecular Weight: 45.5 kDa
• GenBank Accession Number: BC012291
• Gene Symbol: ASCC1
• Gene ID (NCBI): 51008
• RRID: AB_2059350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASCC1 (also known as p50) is a subunit of an uncharacterized complex, the activating signal co-integrator complex (ASCC). ASCC1 has been reported as regulator fo NF-kB pathway and potently inhibits the expression of NF-kB target genes. Mutations in ASCC1 in patients with Barrett esophagus (BE) have been identified as a risk factor of esophageal adenocarcinoma (EAC).

Publications

Species	Application	Title
human	WB	Nat Commun; Extended DNA threading through a dual-engine motor module of the activating signal co-integrator 1 complex; Authors - Junqiao Jia
human	WB	Mol Cell; Translation stress and collided ribosomes are co-activators of cGAS.; Authors - Li Wan
human	WB	Mol Cell; Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.; Authors - Mark Stoneley