ASC/TMS1 Monoclonal antibody, PBS Only

ASC/TMS1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G12E6

Cat no : 67494-1-PBS

Synonyms

PYCARD, hASC, Caspase recruitment domain-containing protein 5, CARD5, ASC

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Validation Data Gallery

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  WB analysis using 67494-1-Ig (same clone as 67494-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 67494-1-Ig (same clone as 67494-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HL-60 using 67494-1-Ig (same clone as 67494-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67494-1-Ig (ASC/TMS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67494-1-PBS in a different storage buffer formulation.

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Product Information

67494-1-PBS targets ASC/TMS1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASC/TMS1 fusion protein Ag28424
• Full Name: PYD and CARD domain containing
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 22-25 kDa
• GenBank Accession Number: BC004470
• Gene Symbol: ASC/TMS1
• Gene ID (NCBI): 29108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PYCARD , also known as ASC or TMS1, is a 195 amino acid protein containing an N-terminal Pyrin-like domain (PYD) and an 87 residue C-terminal Caspase-associated recruitment domain (CARD). It promotes caspase-mediated apoptosis which is mediated predominantly through the activation of caspase-9.