ASB5 Polyclonal antibody

ASB5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24031-1-AP

Synonyms

ASB-5, ASB 5

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Validation Data Gallery

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  WB analysis using 24031-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24031-1-AP (ASB5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 24031-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24031-1-AP (ASB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 24031-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 24031-1-AP (ASB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 24031-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 24031-1-AP (ASB5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 24031-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ASB5 antibody (24031-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse heart tissue, rat skeletal muscle tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24031-1-AP targets ASB5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ASB5 fusion protein Ag21230
• Full Name: ankyrin repeat and SOCS box-containing 5
• Calculated Molecular Weight: 329 aa, 36 kDa
• Observed Molecular Weight: 36-40 kDa
• GenBank Accession Number: BC065710
• Gene Symbol: ASB5
• Gene ID (NCBI): 140458
• RRID: AB_2879410
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ASB5, also named as Ankyrin repeat and SOCS box-containing 5, is a 329 amino acid protein, which contains a conserved one C-terminal SOCS box motif. Asb5 was significantly upregulated in growing collateral arteries on mRNA and protein level. It has been shown that asb5 is a protein implicated in the initiation of arteriogenesis.