Beta Arrestin 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Beta Arrestin 1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 15361-1-AP

Synonyms

ARB1, ARR1, ARRB1, Arrestin beta 1, arrestin, beta 1, Beta arrestin 1, β-Arrestin-1

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Validation Data Gallery

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  WB analysis using 15361-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15361-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 15361-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 15361-1-AP (ARRB1 antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 15361-1-AP

  IP result of anti-Beta Arrestin 1 (IP:15361-1-AP, 4ug; Detection:15361-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human tonsillitis using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP (beta Arrestin 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15361-1-AP (beta Arrestin 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15361-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15361-1-AP (Beta Arrestin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, U-937 cells, rat lung tissue, mouse lung tissue, RAW 264.7 cells
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human tonsillitis tissue, mouse spleen tissue, mouse cerebellum tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 9 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

15361-1-AP targets Beta Arrestin 1 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Beta Arrestin 1 fusion protein Ag7608
• Full Name: arrestin, beta 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47-55 kDa
• GenBank Accession Number: BC003636
• Gene Symbol: Beta Arrestin 1
• Gene ID (NCBI): 408
• RRID: AB_2060227
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

β-Arrestins (ARRBs), the best known regulators of G protein-coupled receptor signaling, are versatile and multifunctional adapter proteins that regulate diverse cellular functions, including cell growth, apoptosis and immune responses. Overexpression of beta Arrestin 1 has been found in various cancers, indicating it as a potential therapeutic target for cancer treatment. Recently expression of ARRB1 in saliva has been identified as a candidate circadian biomarker. ARRB1 migrated as a doublet of two bands of 45 and 55 kDa (PMID:28947386).

Publications

Species	Application	Title
human,mouse	WB,CoIP	Cell Metab; Autonomous sensing of the insulin peptide by an olfactory G protein-coupled receptor modulates glucose metabolism.; Authors - Jie Cheng ; KD Validated
mouse	IF	J Cell Biol; BBSome trains remove activated GPCRs from cilia by enabling passage through the transition zone.; Authors - Fan Ye
	IF	Cell Death Dis; β-Arrestins promote podocyte injury by inhibition of autophagy in diabetic nephropathy.; Authors - J Liu
human	WB	J Biol Chem; An ionic lock and a hydrophobic zipper mediate the coupling between an insect pheromone receptor BmOR3 and downstream effectors.; Authors - Jing-Yu Lin ; KD Validated
human	WB	PLoS One; Cell Survival Following Radiation Exposure Requires miR-525-3p Mediated Suppression of ARRB1 and TXN1.; Authors - Kraemer Anne A
mouse	IF	Oncol Rep; Blue light irradiation inhibits the growth of colon cancer and activation of cancer‑associated fibroblasts.; Authors - Toshiaki Yoshimoto