Arrestin C Polyclonal antibody

Arrestin C Polyclonal Antibody for WB, IHC, IF, ELISA
Cat No. 11100-2-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Arrestin-C, ARR3, Retinal cone arrestin-3, C-arrestin, CAR

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inhuman brain tissue, mouse eye tissue, mouse heart tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRetinal organoids, mouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11100-2-AP targets Arrestin C in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Arrestin C fusion protein Ag1580 Predict reactive species
Full Name arrestin 3, retinal (X-arrestin)
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC012096
Gene Symbol Arrestin C
Gene ID (NCBI) 407
RRIDAB_2289959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36575
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Arrestin C, also known as Arrestin 3 or Retinal Cone Arrestin, is a protein encoded by the ARR3 gene. It belongs to the arrestin family, which plays a crucial role in regulating G-protein-coupled receptor (GPCR) signaling and trafficking. Arrestin C is composed of two major domains: the N-domain and the C-domain, connected by a hinge region. These domains form a structure resembling two clamshells placed end-to-end. The C-terminal tail (C-tail) of Arrestin C interacts extensively with the N-domain, stabilizing its basal conformation. Arrestin C is predominantly expressed in cone photoreceptors and pinealocytes in the retina. It is involved in the shut-off mechanisms associated with high-acuity color vision by binding to phosphorylated and activated opsins, thereby inhibiting their ability to interact with transducin.

Protocols

Product Specific Protocols
WB protocol for Arrestin C antibody 11100-2-APDownload protocol
IHC protocol for Arrestin C antibody 11100-2-APDownload protocol
IF protocol for Arrestin C antibody 11100-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

A high-risk retinoblastoma subtype with stemness features, dedifferentiated cone states and neuronal/ganglion cell gene expression.

Authors - Jing Liu
humanIHC

Cancers (Basel)

Clinical, Genomic, and Pharmacological Study of MYCN-Amplified RB1 Wild-Type Metastatic Retinoblastoma.

Authors - Santiago Zugbi
humanIHC

Int J Mol Sci

Tridimensional Retinoblastoma Cultures as Vitreous Seeds Models for Live-Cell Imaging of Chemotherapy Penetration.

Authors - Ursula Winter
mouseWB

Front Mol Neurosci

A mouse model of cone photoreceptor function loss (cpfl9) with degeneration due to a mutation in Gucy2e

Authors - Anna S E N Naggert
humanIHC

Pediatr Blood Cancer

Immunohistochemical expression of TFF1 is a marker of poor prognosis in retinoblastoma

Authors - Rosario Aschero
humanIHC

Invest Ophthalmol Vis Sci

Establishment and Comprehensive Characterization of a Novel Preclinical Platform of Metastatic Retinoblastoma for Therapeutic Developments

Authors - Santiago Zugbi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Alessandro (Verified Customer) (01-19-2023)

great outcome, no unspecific staining

  • Applications: Immunofluorescence
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