ARPC5L Polyclonal antibody, PBS Only

ARPC5L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 22025-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 22025-1-AP (same clone as 22025-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 22025-1-AP (same clone as 22025-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 22025-1-AP (same clone as 22025-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 22025-1-AP (same clone as 22025-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 22025-1-AP (ARPC5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 22025-1-AP (same clone as 22025-1-PBS)

  IP result of anti-ARPC5L (IP:22025-1-AP, 4ug; Detection:22025-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 22025-1-AP (same clone as 22025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 22025-1-AP (same clone as 22025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 22025-1-AP (same clone as 22025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cerebellum using 22025-1-AP (same clone as 22025-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cerebellum using 22025-1-AP (ARPC5L antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 22025-1-AP (same clone as 22025-1-PBS)

  Immunofluorescent analysis of MCF-7 cells using 22025-1-AP (ARPC5L antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 22025-1-PBS in a different storage buffer formulation.

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Product Information

22025-1-PBS targets ARPC5L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARPC5L fusion protein Ag16881
• Full Name: actin related protein 2/3 complex, subunit 5-like
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17-20 kDa
• GenBank Accession Number: BC000798
• Gene Symbol: ARPC5L
• Gene ID (NCBI): 81873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ARPC5L, also known as ARC16-2, belongs to the ARPC5 family. ARPC5L is involved in Arp2/3 complex-mediated actin nucleation and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. ARPC5L-containing Arp2/3 complexes are required for the peripheral positioning of nuclei in myofibers (PMID: 28892082). The molecular mass of ARPC5L is 17-20 kDa.