ARPC3 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARPC3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 14652-1-AP

Synonyms

ARC21, Arp2/3 complex 21 kDa subunit, ARPC3

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Validation Data Gallery

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  WB analysis of HeLa using 14652-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14652-1-AP (ARPC3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 14652-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14652-1-AP (ARPC3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 14652-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14652-1-AP (ARPC3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 14652-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14652-1-AP (ARPC3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 14652-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14652-1-AP (ARPC3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, mouse brain, rat brain
• Positive IHC detected in: human colon cancer tissue, mouse spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14652-1-AP targets ARPC3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARPC3 fusion protein Ag6249
• Full Name: actin related protein 2/3 complex, subunit 3, 21kDa
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC067747
• Gene Symbol: ARPC3
• Gene ID (NCBI): 10094
• RRID: AB_2059933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Immunol; Heme drives hemolysis-induced susceptibility to infection via disruption of phagocyte functions.; Authors - Rui Martins
human	WB	J Cell Biol; α5β1 integrin recycling promotes Arp2/3-independent cancer cell invasion via the formin FHOD3.; Authors - Nikki R Paul ; KD Validated
human	IP	EMBO Rep; WDR63 inhibits Arp2/3-dependent actin polymerization and mediates the function of p53 in suppressing metastasis.; Authors - Kailiang Zhao
human	WB	Cell Rep; Nuclear translocation of cGAS orchestrates VEGF-A-mediated angiogenesis; Authors - Juanjuan Luo ; KO Validated
mouse,bovine	WB,IF	Biomolecules; Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model; Authors - Bo Fang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kiara (Verified Customer) (11-15-2021)

Works great for Western Blot! We used 5% milk in TBS-T to block the membrane and diluted the ARPC3 antibody 1:1000 in 5% milk in TBS-T. We received one specific clear band. Awesome antibody!

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Neonatal microglia