BMAL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BMAL1 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

61

Cat no : 14268-1-AP

Synonyms

ARNTL, ARNT3, bHLHe5, Basic-helix-loop-helix-PAS protein MOP3, Basic helix-loop-helix ARNT-like protein 1

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Validation Data Gallery

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  WB analysis using 14268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14268-1-AP (BMAL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14268-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14268-1-AP (BMAL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 14268-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 14268-1-AP (ARNTL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 14268-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 14268-1-AP (ARNTL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14268-1-AP

  IP result of anti-BMAL1 (IP:14268-1-AP, 4ug; Detection:14268-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IF Staining of U-87 MG using 14268-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using BMAL1 antibody (14268-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  FC experiment of HeLa using 14268-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human ARNTL (14268-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HT-1080 cells, 3T3-L1 cells, HeLa cells, MCF-7 cells, NCI-H1299 cells, mouse liver tissue, rat liver tissue, SH-SY5Y cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: U-87 MG cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 51 publications below
• IHC: See 14 publications below
• IF: See 8 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 5 publications below

Product Information

14268-1-AP targets BMAL1 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BMAL1 fusion protein Ag5586
• Full Name: aryl hydrocarbon receptor nuclear translocator-like
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 69-75 kDa
• GenBank Accession Number: BC041129
• Gene Symbol: ARNTL
• Gene ID (NCBI): 406
• RRID: AB_2878037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BMAL1, a basic helix-loop-helices with Per-Arnt-Sim (PAS) domain transcription factor, is one of components that has core clock function in mammals. It form a heterodimer with CLOCK, and induce the transcription of target genes by binding to E-box elements within their promoter regions. And this transcription is inhibited in a feedback loop by PER, and also by CRY proteins.Also BMAL1 is a key player in the regulation of metabolism, playing a role in adipogenesis and in the control of glucose and triglycerides levels. Due to phosphorylation modification, BMAL1 can be detected as a band in the range of 70-80 kda (PMID: 14750952).

Publications

Species	Application	Title
human	WB	Mol Cell; CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis.; Authors - Ran Lin ; KD Validated
human	WB	Nat Commun; The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.; Authors - Yi Liu
mouse	WB	J Pineal Res; Circadian misalignment impairs oligodendrocyte myelination via Bmal1 overexpression leading to anxiety and depression-like behaviors; Authors - Yao Zuo
	WB	Sci Total Environ; Metastatic effects of perfluorooctanoic acid (PFOA) on Drosophila melanogaster with metabolic reprogramming and dysrhythmia in a multigenerational exposure scenario; Authors - Fangnon Firmin Fangninou
mouse	IHC	Theranostics; Circadian Clock Gene Bmal1 Regulates Bilirubin Detoxification: A Potential Mechanism of Feedback Control of Hyperbilirubinemia.; Authors - Shuai Wang
mouse	WB	Environ Pollut; Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction.; Authors - Feifei Shi