ARMCX3 Polyclonal antibody

• Featured Product
• KD/KO Validated

ARMCX3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25705-1-AP

Synonyms

UNQ2517/PRO6007, Protein ALEX3, KIAA0443, BM-017, Armadillo repeat-containing X-linked protein 3

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Validation Data Gallery

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  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25705-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25705-1-AP (ARMCX3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of fetal human brain using 25705-1-AP

  IP result of anti-ARMCX3 (IP:25705-1-AP, 1:500; Detection:25705-1-AP, 1:3000) with human brain lysate, 150 ug. Courtesied by Dr. Mirra and Dr. Manso of University of Barcelona.

• 

  IHC staining of human ovary cancer using 25705-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25705-1-AP (ARMCX3 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IP detected in: fetal human brain
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below

Product Information

25705-1-AP targets ARMCX3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARMCX3 fusion protein Ag22501
• Full Name: armadillo repeat containing, X-linked 3
• Calculated Molecular Weight: 379 aa, 43 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC005194
• Gene Symbol: ARMCX3
• Gene ID (NCBI): 51566
• RRID: AB_2880201
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARMCX3, also named as Armadillo repeat-containing X-linked protein 3, is a 379 amino acid protein, which contains 3 ARM repeats. ARMCX3 is a single pass membrane protein belonging to the armadillo repeat family of proteins. ARMCX3 is believed to play a role in embryonic development and tissue maintenance and may also function as a tumor suppressor.

Publications

Species	Application	Title
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KD Validated
	WB	Sci Signal; A mammalian-specific Alex3/Gαq protein complex regulates mitochondrial trafficking, dendritic complexity, and neuronal survival; Authors - Ismael Izquierdo-Villalba ; KO Validated
human	WB	Cancers (Basel); ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity.; Authors - Serena Mirra
mouse	WB	Int J Obes (Lond); The armadillo-repeat containing X-linked protein 3, ARMCX3, is a negative regulator of the browning of adipose tissue associated with obesity.; Authors - Aleix Gavaldà-Navarro

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