ARL8B Monoclonal antibody

ARL8B Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G10E2

Cat no : 67891-1-Ig

Synonyms

ARL10C, ARL8B, FLJ10702, Gie1

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Validation Data Gallery

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  WB analysis using 67891-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of LNCaP using 67891-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67891-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67891-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67891-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 67891-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67891-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67891-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67891-1-Ig (ARL8B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A549 using 67891-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using ARL8B antibody (67891-1-Ig, Clone: 2G10E2 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells, pig brain tissue, rat brain tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67891-1-Ig targets ARL8B in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARL8B fusion protein Ag3711
• Full Name: ADP-ribosylation factor-like 8B
• Calculated Molecular Weight: 186 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC013131
• Gene Symbol: ARL8B
• Gene ID (NCBI): 55207
• RRID: AB_2918647
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.