ARL8B Polyclonal antibody

• Featured Product
• KD/KO Validated

ARL8B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

24

Reviews (1)

Cat no : 13049-1-AP

Synonyms

GIE1, EC:3.6.5.2, ARL10C, ADP-ribosylation factor-like protein 8B, ADP-ribosylation factor-like protein 10C

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Validation Data Gallery

• 

  WB analysis of fetal human brain using 13049-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13049-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13049-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13049-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13049-1-AP (ARL8B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 13049-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13049-1-AP (ARL8B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 13049-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ARL8B antibody (13049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, human brain tissue, mouse brain tissue, NIH/3T3 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 9 publications below
• CoIP: See 1 publications below

Product Information

13049-1-AP targets ARL8B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, monkey, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARL8B fusion protein Ag3711
• Full Name: ADP-ribosylation factor-like 8B
• Calculated Molecular Weight: 186 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC013131
• Gene Symbol: ARL8B
• Gene ID (NCBI): 55207
• RRID: AB_2059000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL8B ( ADP-ribosylation factor-like 8B) , also known as ARL10C or GIE1, is a small GTPase of the Arl-family, that is associated with the lysosome. ARL8A interacts with Tubulin, and plays a role in lysosome motility, as well as in chromosomal segregation. ARL8B plays a role in the spatial distribution and positioning of lysosomes. ARL8B is regarded as a regulator of endosome to lysosome trafficking pathways of special significance for host defense.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Lysosomal positioning coordinates cellular nutrient responses.; Authors - Korolchuk Viktor I VI ; KD Validated
human	WB	Nat Commun; DENND6A links Arl8b to a Rab34/RILP/dynein complex, regulating lysosomal positioning and autophagy; Authors - Rahul Kumar
monkey	WB	Sci Adv; The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes.; Authors - Meng Lu ; KD Validated
mouse	WB,IF	Sci Adv; Endosomal sorting drives the formation of axonal prion protein endoggresomes.; Authors - Romain Chassefeyre ; KO Validated
human	WB	Nat Commun; RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin.; Authors - Tal Keren-Kaplan
mouse	WB	J Cell Biol; The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration.; Authors - Natalia Schiefermeier

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Olivia (Verified Customer) (01-08-2020)

WB: Lysates from mouse bone marrow-derived macrophages were subjected to SDS PAGE followed by western blot with 13049-1-AP(Arl8b antibody) at a dilution of 1:500 overnight at 4°C.IF: Mouse bone marrow-derived macrophages were fixed in 4% PFA and permeabilized with 0.5% saponin before incubation with 1:500 Arl8b antibody (in 1% BSA/0.1% saponin/PBS) overnight at 4°C, followed by incubation with anti-rabbit Alexa Fluor 568 for 1h at room temperature.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse bone marrow-derived macrophages

ARL8B Antibody Western Blot,Immunofluorescence, validation (1:500 dilution) in Mouse bone marrow-derived macrophages (Cat no:13049-1-AP)

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