ARL5B Polyclonal antibody

• Featured Product
• KD/KO Validated

ARL5B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11694-1-AP

Synonyms

ARL8, ADP-ribosylation factor-like protein 8, ADP-ribosylation factor-like protein 5B

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Validation Data Gallery

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  WB analysis of U2OS using 11694-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 11694-1-AP (ARL5B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 11694-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11694-1-AP (ARL5B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 11694-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11694-1-AP (ARL5B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U2OS cells, HepG2 cells, HeLa cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Product Information

11694-1-AP targets ARL5B in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARL5B fusion protein Ag2309
• Full Name: ADP-ribosylation factor-like 5B
• Calculated Molecular Weight: 179 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC024163
• Gene Symbol: ARL5B
• Gene ID (NCBI): 221079
• RRID: AB_2058835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL5B, also known as ADP ribosylation factor-like GTPase 5B, is a small G protein that is localized to the trans-Golgi network (TGN) in mammalian cells. It plays a significant role in the regulation of retrograde membrane transport from endosomes back to the TGN. This function is particularly important for the maintenance of cellular membrane traffic and the organization of the Golgi apparatus.

Publications

Species	Application	Title
human	WB, IP	J Biol Chem; Negative regulation of MDA5-dependent antiviral innate immune responses by Arl-like protein 5B.; Authors - Yuichi Kitai ; KO Validated
mouse	WB	J Cell Biochem; GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis.; Authors - Chun-Xiang Zhou