ARL3 Monoclonal antibody

ARL3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, rabbit

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E5B12

Cat no : 68421-1-Ig

Synonyms

ADP ribosylation factor like 3, ARFL3, ARL3

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Validation Data Gallery

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  WB analysis of HeLa using 68421-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit testis using 68421-1-Ig

  rabbit testis tissue were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat retina using 68421-1-Ig

  rat retina tissue were subjected to SDS PAGE followed by western blot with 68421-1-Ig (ARL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of hTERT-RPE1 using 68421-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL3 antibody (68421-1-Ig, Clone: 2E5B12 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

• Positive WB detected in: HeLa cells, rabbit testis tissue, rat retina tissue
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68421-1-Ig targets ARL3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat, rabbit samples.

• Tested Reactivity: Human, rat, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ARL3 fusion protein Ag21387
• Full Name: ADP-ribosylation factor-like 3
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 20-24 kDa
• GenBank Accession Number: BC009841
• Gene Symbol: ARL3
• Gene ID (NCBI): 403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL3 (ADP-ribosylation factor-like 3) is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. ARL3 is believed to be involved in ciliary and microtubule-dependent processes.