ARL13B Polyclonal antibody

ARL13B Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Guinea Pig / IgG

Reactivity

Human

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 30332-1-AP

Synonyms

ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8

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Validation Data Gallery

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  WB analysis using 30332-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30332-1-AP (ARL13B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The second antibody is used against guinea pigs.

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  IP experiment of L02 using 30332-1-AP

  IP result of anti-ARL13B (IP:30332-1-AP, 4ug; Detection:30332-1-AP 1:4000) with L02 cells lysate 3080 ug.

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  IF Staining of hTERT-RPE1 using 30332-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using ARL13B antibody (30332-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, green).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, K-562 cells, L02 cells
• Positive IP detected in: L02 cells
• Positive IF detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30332-1-AP targets ARL13B in WB, IP, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Guinea Pig / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARL13B fusion protein Ag12015
• Full Name: ADP-ribosylation factor-like 13B
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC094725
• Gene Symbol: ARL13B
• Gene ID (NCBI): 200894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870).This antibody detects two specific bands at 60 kDa and 48 kDa. Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986). The second antibody is used against guinea pigs.