ARK5 Recombinant antibody

ARK5 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242411F1

Cat no : 84893-5-RR

Synonyms

NUAK1, NUAK family SNF1-like kinase 1, NUAK 1, EC:2.7.11.1, AMPK-related protein kinase 5

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Validation Data Gallery

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  IF Staining of A431 using 84893-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using NUAK1 antibody (84893-5-RR, Clone: 242411F1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84893-5-RR targets ARK5 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ARK5 fusion protein Ag18832
• Full Name: NUAK family, SNF1-like kinase, 1
• Calculated Molecular Weight: 661 aa, 74 kDa
• GenBank Accession Number: BC152462
• Gene Symbol: ARK5
• Gene ID (NCBI): 9891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NUAK1 is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. It is also named as ARK5, KIAA0537, OMPHK1 and belongs to the protein kinase superfamily. The physiological role of NUAK1 is potentially to suppress glucose uptake through negative regulation of INS signaling in oxidative muscle. This protein has 2 isoforms produced by alternative splicing.