ARK5 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ARK5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 22723-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 22723-1-AP (same clone as 22723-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 22723-1-AP (same clone as 22723-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22723-1-AP (ARK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22723-1-AP (same clone as 22723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 22723-1-AP (same clone as 22723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 22723-1-AP (same clone as 22723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 22723-1-AP (same clone as 22723-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22723-1-AP (ARK5 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22723-1-PBS in a different storage buffer formulation.

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Product Information

22723-1-PBS targets ARK5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARK5 fusion protein Ag18612
• Full Name: NUAK family, SNF1-like kinase, 1
• Calculated Molecular Weight: 661 aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC152462
• Gene Symbol: ARK5
• Gene ID (NCBI): 9891
• RRID: AB_2879157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

NUAK1 is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. It is also named as ARK5, KIAA0537, OMPHK1 and belongs to the protein kinase superfamily. The physiological role of NUAK1 is potentially to suppress glucose uptake through negative regulation of INS signaling in oxidative muscle. This protein has 2 isoforms produced by alternative splicing.