ARID4B Polyclonal antibody

• Featured Product
• KD/KO Validated

ARID4B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 24499-1-AP

Synonyms

RBBP1L1, Breast cancer-associated antigen BRCAA1, BRCAA1, AT-rich interactive domain-containing protein 4B, ARID domain-containing protein 4B

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Validation Data Gallery

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  WB analysis of MCF-7 using 24499-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 24499-1-AP (ARID4B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 24499-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24499-1-AP (ARID4B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 24499-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 24499-1-AP (ARID4B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 24499-1-AP

  IP result of anti-ARID4B (IP:24499-1-AP, 5ug; Detection:24499-1-AP 1:1000) with MCF-7 cells lysate 4000ug.

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  IHC staining of human pancreas cancer using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24499-1-AP (ARID4B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24499-1-AP (ARID4B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24499-1-AP (ARID4B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24499-1-AP (ARID4B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24499-1-AP (ARID4B Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 24499-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24499-1-AP (ARID4B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 24499-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ARID4B antibody (24499-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: MCF-7 cells, A549 cells, K-562 cells
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human pancreas cancer tissue, human breast cancer tissue, human cervical cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Product Information

24499-1-AP targets ARID4B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARID4B fusion protein Ag21462
• Full Name: AT rich interactive domain 4B (RBP1-like)
• Calculated Molecular Weight: 1312 aa, 148 kDa
• Observed Molecular Weight: 100 kDa, 200 kDa
• GenBank Accession Number: BC130418
• Gene Symbol: ARID4B
• Gene ID (NCBI): 51742
• RRID: AB_2879575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARID4B, also named as BRCAA1 or SAP180, is a 1312 amino acid protein, which contains one ARID domain. ARID4B localizes in the nucleus and cytoplasm. ARID4B as a transcription repressor may function in the assembly and/or enzymatic activity of the Sin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. ARID4B is highly expressed in the testis and in breast, lung, colon, pancreatic and ovarian cancers. Expressed at low levels in the thymus, prostate and ovary.

Publications

Species	Application	Title
human	WB	J Clin Invest; The FOXN3-NEAT1-SIN3A repressor complex promotes progression of hormonally responsive breast cancer.; Authors - Wanjin Li
human	WB	Theranostics; SLC14A1 prevents oncometabolite accumulation and recruits HDAC1 to transrepress oncometabolite genes in urothelial carcinoma.; Authors - Ti-Chun Chan
human	WB	Cell Death Dis; DNA-methylation-mediated silencing of miR-486-5p promotes colorectal cancer proliferation and migration through activation of PLAGL2/IGF2/β-catenin signal pathways.; Authors - Xiangxiang Liu
mouse	WB	Br J Nutr; Taurine stimulates protein synthesis and proliferation of C2C12 myoblast cells through the PI3K-ARID4B-mTOR pathway.; Authors - Qi Hao ; KD Validated
human	WB,IP	Cancer Lett; Forkhead Box Protein FOXK1 Disrupts the Circadian Rhythm to Promote Breast Tumorigenesis in Response to Insulin Resistance; Authors - Zhaohan Zhang