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ARID4A Recombinant antibody

ARID4A Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240436C9

Cat no : 83489-3-RR

Synonyms

RBP1, RBP 1, RBBP1, RBBP 1, ARID 4A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARID4A Recombinant antibody (83489-3-RR)

    WB analysis using 83489-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83489-3-RR (ARID4A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83489-3-RR

    Affinity and Kinetic Characterization of 83489-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83489-3-RR against Human ARID4A were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83489-3-RR is ARID4A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARID4A fusion protein Ag33114
Full Name AT rich interactive domain 4A (RBP1-like)
Calculated Molecular Weight143 kDa
Observed Molecular Weight205 kDa
GenBank Accession NumberNM_002892.3
Gene Symbol ARID4A
Gene ID (NCBI) 5926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARID4A (AT-rich interaction domain 4A, also known as retinoblastoma-binding protein 1, RBBP1/RBP1) is a retinoblastoma (RB)-binding protein. ARID1A is an important subunit of the switching defective/sucrose nonfermenting (SWI/SNF) chromatin remodeling complex.

Protocols

Product Specific Protocols
WB protocol for ARID4A antibody 83489-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols