ARID2 Polyclonal antibody

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  WB analysis using 23406-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23406-1-AP (ARID2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 23406-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23406-1-AP (ARID2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 23406-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 23406-1-AP (ARID2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARID2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 23406-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Zinc finger protein with activation potential, KIAA1557, BRG1-associated factor 200, BAF200, AT-rich interactive domain-containing protein 2

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

23406-1-AP targets ARID2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARID2 fusion protein Ag19968
• Full Name: AT rich interactive domain 2 (ARID, RFX-like)
• Calculated Molecular Weight: 1835 aa, 197 kDa
• Observed Molecular Weight: 200-230 kDa
• GenBank Accession Number: BC090062
• Gene Symbol: ARID2
• Gene ID (NCBI): 196528
• RRID: AB_2918080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q68CP9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Am Heart Assoc; Aging-Associated ALKBH5-m6A Modification Exacerbates Doxorubicin-Induced Cardiomyocyte Apoptosis Via AT-Rich Interaction Domain 2; Authors - Runtai Chen