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ARID1A Recombinant antibody, PBS Only (Capture)

ARID1A Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240690F1

Cat no : 83733-3-PBS

Synonyms

BAF250A, BAF250, B120, AT-rich interactive domain-containing protein 1A, ARID domain-containing protein 1A

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using ARID1A Recombinant antibody (83733-3-RR)

    WB analysis of BxPC-3 using 83733-3-RR (same clone as 83733-3-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ARID1A Recombinant antibody (83733-3-RR)

    WB analysis using 83733-3-RR (same clone as 83733-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83733-3-RR (ARID1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARID1A Recombinant antibody (83733-3-RR)

    IF Staining of HeLa using 83733-3-RR (same clone as 83733-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ARID1A Recombinant antibody (83733-3-RR)

    IF Staining of HeLa using 83733-3-RR (same clone as 83733-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ARID1A antibody (83733-3-RR, Clone: 240690F1 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83733-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00714-2

    Cytometric bead array standard curve of MP00714-2

    Cytometric bead array standard curve of MP00714-2, ARID1A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83733-3-PBS. Detection antibody: 83733-2-PBS. Standard: Ag30749. Range: 1.25 -80 ng/mL.

  • Affinity and Kinetic Characterization of 83733-3-RR

    Affinity and Kinetic Characterization of 83733-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83733-3-RR against Human ARID1A were performed. The affinity constant is 0.462 nM.

Product Information

83733-3-PBS targets ARID1A as part of a matched antibody pair:

MP00714-2: 83733-3-PBS capture and 83733-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ARID1A fusion protein Ag30749
Full Name AT rich interactive domain 1A (SWI-like)
Calculated Molecular Weight 242 kDa
Observed Molecular Weight250-260 kDa
GenBank Accession NumberNM_006015
Gene Symbol ARID1A
Gene ID (NCBI) 8289
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.