ARID1A Polyclonal antibody

ARID1A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 30304-1-AP

Synonyms

ARID1A, B120, BAF250, BAF250a, BM029, BRG1 associated factor 250, BRG1 associated factor 250a, C1orf4, hELD, hOSA1, Osa homolog 1, OSA1, P270, SMARCF1, SWI like protein, SWI/SNF complex protein p270

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Validation Data Gallery

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  WB analysis using 30304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30304-1-AP (ARID1A antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 30304-1-AP

  IP result of anti-ARID1A (IP:30304-1-AP, 4ug; Detection:30304-1-AP 1:3000) with HeLa cells lysate 1280 ug.

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  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 30304-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30304-1-AP (ARID1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, K-562 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human endometrial cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30304-1-AP targets ARID1A in WB, IP, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARID1A fusion protein Ag30749
• Full Name: AT rich interactive domain 1A (SWI-like)
• Calculated Molecular Weight: 242 kDa
• Observed Molecular Weight: 250-260 kDa
• GenBank Accession Number: NM_006015
• Gene Symbol: ARID1A
• Gene ID (NCBI): 8289
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARID1A, also named as BAF250, BAF250A, C1orf4, OSA1 and SMARCF1, is involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). It binds DNA non-specifically. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. ARID1A belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The antibody is specific to ARID1A.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Roy (Verified Customer) (06-15-2024)

Great antibody that detects ARID1A by WB from HeLa extracts (1/1000 dilution, 1h at room temperature)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa