WB analysis of MCF-7 using 11379-1-AP (same clone as 11379-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
MCF7 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of human kidney using 11379-1-AP (same clone as 11379-1-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
human kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of human liver using 11379-1-AP (same clone as 11379-1-PBS)
human liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
human liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 11379-1-AP (same clone as 11379-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 11379-1-AP (same clone as 11379-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 11379-1-AP (same clone as 11379-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 11379-1-AP (same clone as 11379-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
HeLa cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of rat kidney using 11379-1-AP (same clone as 11379-1-PBS)
rat kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
rat kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
IP experiment of mouse kidney using 11379-1-AP (same clone as 11379-1-PBS)
IP result of anti-ARHGEF5 (IP:11379-1-AP, 4ug; Detection:11379-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
IP result of anti-ARHGEF5 (IP:11379-1-AP, 4ug; Detection:11379-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 11379-1-AP (same clone as 11379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 11379-1-AP (same clone as 11379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 11379-1-AP (same clone as 11379-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11379-1-AP (ARHGEF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11379-1-AP (ARHGEF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
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WB analysis of MCF-7 using 11379-1-AP (same clone as 11379-1-PBS)
MCF7 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of human kidney using 11379-1-AP (same clone as 11379-1-PBS)
human kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of human liver using 11379-1-AP (same clone as 11379-1-PBS)
human liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 11379-1-AP (same clone as 11379-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of mouse liver using 11379-1-AP (same clone as 11379-1-PBS)
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of mouse kidney using 11379-1-AP (same clone as 11379-1-PBS)
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 11379-1-AP (same clone as 11379-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
WB analysis of rat kidney using 11379-1-AP (same clone as 11379-1-PBS)
rat kidney tissue were subjected to SDS PAGE followed by western blot with 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of human lung cancer using 11379-1-AP (same clone as 11379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 11379-1-AP (same clone as 11379-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11379-1-AP (ARHGEF5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
IP Figures
IP experiment of mouse kidney using 11379-1-AP (same clone as 11379-1-PBS)
IP result of anti-ARHGEF5 (IP:11379-1-AP, 4ug; Detection:11379-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HeLa using 11379-1-AP (same clone as 11379-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11379-1-AP (ARHGEF5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11379-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
ARHGEF5 Polyclonal antibody
Catalog Number
11379-1-PBS
Citations
-
Dilutions
Applications
WB, IHC, IF/ICC, IP, Indirect ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
