ARHGDIB Polyclonal antibody

• Featured Product
• KD/KO Validated

ARHGDIB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 16122-1-AP

Synonyms

ARHGDIB, D4, GDIA2, GDID4, Ly GDI, LYGDI, RAP1GN1, Rho GDI 2, Rho GDI beta, RhoGDI2

---

Validation Data Gallery

• 

  WB analysis using 16122-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16122-1-AP (ARHGDIB antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16122-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16122-1-AP (ARHGDIB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 16122-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 16122-1-AP (ARHGDIB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 16122-1-AP

  IP result of anti-ARHGDIB (IP:16122-1-AP, 4ug; Detection:16122-1-AP 1:1000) with Raji cells lysate 2000ug.

• 

  IHC staining of human stomach cancer using 16122-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16122-1-AP (ARHGDIB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human stomach cancer using 16122-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 16122-1-AP (ARHGDIB antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, rat spleen tissue, Jurkat cells, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

16122-1-AP targets ARHGDIB in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARHGDIB fusion protein Ag9078
• Full Name: Rho GDP dissociation inhibitor (GDI) beta
• Calculated Molecular Weight: 201 aa, 23 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC009200
• Gene Symbol: ARHGDIB
• Gene ID (NCBI): 397
• RRID: AB_2059596
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGDIB, also known as RhoGDI2, is a conserved member of the RhoGDI family and plays an important role in cell migrations. It regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. It is mainly in hematopoietic, endothelial, and epithelial cells. It has been linked to tumorigenesis and metastasis. RhoGDI2 expression is downregulated in several cancer types, such as bladder, lung and lymphoma, but is upregulated in prostate and gastric cancer.

Publications

Species	Application	Title
human	WB	Nat Metab; Enhanced BCAT1 activity and BCAA metabolism promotes RhoC activity in cancer progression; Authors - Lin Qian
human	WB	Nat Immunol; Kinases Mst1 and Mst2 positively regulate phagocytic induction of reactive oxygen species and bactericidal activity.; Authors - Jing Geng
human	WB	Mol Cancer; KDM6A-ARHGDIB axis blocks metastasis of bladder cancer by inhibiting Rac1.; Authors - Lei Liu
human	WB	Proteomics; Changes in the proteomic profile during the differential polarization status of the human monocyte-derived macrophage THP-1 cell line.; Authors - Fan Zhang
human	WB,IHC	Cancer Biomark; Systematic investigation of biomarker-like role of ARHGDIB in breast cancer.; Authors - Xiaonan Wang ; KD Validated
human	WB	DNA Cell Biol; Rho GDP Dissociation Inhibitor (GDI) Beta Promotes Cell Proliferation and Invasion by Modulating the AKT Pathway in Hepatocellular Carcinoma.; Authors - Yang Fang