RhoGDI Monoclonal antibody

• Featured Product
• KD/KO Validated

RhoGDI Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B1G9

Cat no : 66480-1-Ig

Synonyms

aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1

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Validation Data Gallery

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  WB analysis using 66480-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66480-1-Ig (ARHGDIA,aGDI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66480-1-Ig

  WB result of RhoGDI antibody (66480-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RhoGDI transfected HEK-293 cells.

• 

  WB analysis using 66480-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66480-1-Ig (ARHGDIA,aGDI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, C6 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66480-1-Ig targets RhoGDI in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RhoGDI fusion protein Ag0791
• Full Name: Rho GDP dissociation inhibitor (GDI) alpha
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC005851
• Gene Symbol: RhoGDI
• Gene ID (NCBI): 396
• RRID: AB_2881846
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.