Tested Applications
Positive WB detected in | HeLa cells, C6 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, NIH/3T3 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
66480-1-Ig targets RhoGDI in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Reactivity | Human, Mouse, Rat |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | RhoGDI fusion protein Ag0791 Predict reactive species |
Full Name | Rho GDP dissociation inhibitor (GDI) alpha |
Calculated Molecular Weight | 28 kDa |
Observed Molecular Weight | 28 kDa |
GenBank Accession Number | BC005851 |
Gene Symbol | RhoGDI |
Gene ID (NCBI) | 396 |
RRID | AB_2881846 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P52565 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.
Protocols
Product Specific Protocols | |
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WB protocol for RhoGDI antibody 66480-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |