ARHGAP5 Polyclonal antibody

ARHGAP5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 55165-1-AP

Synonyms

ARHGAP5, GFI2, p190 B, RhoGAP5

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Validation Data Gallery

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  WB analysis using 55165-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55165-1-AP (ARHGAP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 55165-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55165-1-AP (ARHGAP5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, HepG2 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

55165-1-AP targets ARHGAP5 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Rho GTPase activating protein 5
• Calculated Molecular Weight: 172 kDa
• Observed Molecular Weight: 170-190 kDa
• GenBank Accession Number: NM_001173
• Gene Symbol: ARHGAP5
• Gene ID (NCBI): 394
• RRID: AB_10858787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARHGAP5, also named as RHOGAP5 and p190-B, is GTPase-activating protein for Rho family members. It may play a role in the reduction of the p21rasGTPase-activating potential of p120GAP. ARHGAP5 can be directly phosphorylated on a single identified tyrosine residue by the activated INS and IGF-1 receptors. It also has been shown to be tyrosine phosphorylated by cSrc and vSrc. Additionally the GAP domain of ARHGAP5 is shown to attenuate signal transducing activity of Rac, Rho and CDC42. The antibody is specific to ARHGAP5.

Publications

Species	Application	Title
human	WB	Mol Med Rep; Cucurbitacin I induces apoptosis in ovarian cancer cells through oxidative stress and the p190B‑Rac1 signaling axis.; Authors - Ruli Li
human	WB	Nat Metab; Adipose circular RNAs exhibit dynamic regulation in obesity and functional role in adipogenesis.; Authors - Camille Arcinas

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (01-25-2019)

Good, both for WB and IF.By WB, although there are some non-specific bands, it can detect endogenous protein; by IF, PFA fixation, it labels the protein in the Golgi and the nucleus.

• Applications: Western Blot, Immunofluorescence,