ARFGEF2 Recombinant antibody

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  WB analysis using 84296-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84296-4-RR (ARFGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 84296-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 84296-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84296-4-RR (ARFGEF2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84296-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84296-4-RR against Human ARFGEF2 were performed. The affinity constant is 0.788 nM.

ARFGEF2 Recombinant Antibody for WB, IHC, ELISA

Cat No. 84296-4-RR

Clone No.241538H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

BIG2, Brefeldin A inhibited GEP 2, ARFGEP2, 241538H5

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, HEK-293T cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84296-4-RR targets ARFGEF2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ARFGEF2 fusion protein Ag35396
• Full Name: ADP-ribosylation factor guanine nucleotide-exchange factor 2 (brefeldin A-inhibited)
• Observed Molecular Weight: 202 kDa
• GenBank Accession Number: BC050449
• Gene Symbol: ARFGEF2
• Gene ID (NCBI): 10564
• RRID: AB_3671837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y6D5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 is involved in the activation of ARFs by accelerating replacement of bound GDP with GTP and is involved in Golgi transport. ARFGEF2 contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.