ARFGEF1 Polyclonal antibody

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  WB analysis using 31684-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31684-1-AP (ARFGEF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 31684-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31684-1-AP (ARFGEF1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ARFGEF1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 31684-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

p200 ARF GEP1, P200, Brefeldin A inhibited GEP 1, BIG1, ARFGEP1

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HEK-293T cells, HeLa cells, NIH/3T3 cells, mouse lung tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31684-1-AP targets ARFGEF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARFGEF1 fusion protein Ag35629
• Full Name: ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited)
• Calculated Molecular Weight: 208kDa
• Observed Molecular Weight: 210 kDa
• GenBank Accession Number: NM_001413184
• Gene Symbol: ARFGEF1
• Gene ID (NCBI): 10565
• RRID: AB_3670078
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9Y6D6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ARFGEF1 (ADP ribosylation factor guanine nucleotide exchange factor 1), also known as BIG1 (Brefeldin A-inhibited guanine nucleotide exchange protein 1), is one of the ADP ribosylation factor (ARF) guanine nucleotide exchange factor (GEF) with a conserved catalytic sec7 domain.ARFGEF1 is connected with trafficking regulation through the trans-Golgi network and endosomes (PMID: 35090882).