ARFGAP2 Polyclonal antibody

ARFGAP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16519-1-AP

Synonyms

Nbla10535, IRZ, GTPase-activating protein ZNF289, ARFGAP 2, ARF GAP 2

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 16519-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16519-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16519-1-AP (ARFGAP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 16519-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16519-1-AP (ARFGAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells, mouse brain tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16519-1-AP targets ARFGAP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARFGAP2 fusion protein Ag9740
• Full Name: ADP-ribosylation factor GTPase activating protein 2
• Calculated Molecular Weight: 521 aa, 57 kDa
• Observed Molecular Weight: 57-60 kDa
• GenBank Accession Number: BC030148
• Gene Symbol: ARFGAP2
• Gene ID (NCBI): 84364
• RRID: AB_2878270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.