ARF3 Polyclonal antibody

ARF3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22289-1-AP

Synonyms

ADP-ribosylation factor 3, ADP ribosylation factor 3

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Validation Data Gallery

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  WB analysis of mouse brain using 22289-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22289-1-AP (ARF3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 22289-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22289-1-AP (ARF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 22289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22289-1-AP (ARF3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 22289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 22289-1-AP (ARF3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 22289-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ARF3 antibody (22289-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22289-1-AP targets ARF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ADP-ribosylation factor 3
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: NM_001659
• Gene Symbol: ARF3
• Gene ID (NCBI): 377
• RRID: AB_2879061
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-ribosylation factors (ARFs) are members of the ARF family of GTP-binding proteins of the Ras superfamily, with 20kda protein size. ARFs bind and regulate GTP/GDP cycle by alternating between the active GTP-bound and inactive GDP-bound conformations. ARF family proteins are essential and ubiquitous in eukaryotes. Six highly conserved members of the family have been identified in mammalian cells. They function in vesicular traffic and actin remodelling and other bioprocesses in cells. ARF3 is 95% homolouous to ARF1. Its function was not fully understood. (PMID: 7759471, PMID: 16042562).The antibody is specific to ARF3. If has no cross reaction to other ARF members.