ARCN1 Polyclonal antibody

ARCN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 23843-1-AP

Synonyms

COPD, Delta COP, Delta coat protein, Coatomer subunit delta, archain 1

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Validation Data Gallery

• 

  WB analysis of HepG2 using 23843-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 23843-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 23843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23843-1-AP (ARCN1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 23843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23843-1-AP (ARCN1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 23843-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ARCN1 antibody (23843-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, A549 cells, Jurkat cells, mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

23843-1-AP targets ARCN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ARCN1 fusion protein Ag20836
• Full Name: archain 1
• Calculated Molecular Weight: 511 aa, 57 kDa
• Observed Molecular Weight: 57 kDa, 47 kDa
• GenBank Accession Number: BC093636
• Gene Symbol: ARCN1
• Gene ID (NCBI): 372
• RRID: AB_2879337
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Archain 1 (ARCN1), also known as delta-COP, is a subunit of the coat protein I (COPI) complex, which comprises one of the protein coats that mediate vesicle budding from the membrane. Protein sequences of ARCN1 are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. The gene of ARCN1 has been identified in chromosome band 11q23.3, and sequencing of cDNAs suggests that at least two forms of mRNA with alternative 5' ends are present. (PMID: 7782067; 20502676)

Publications

Species	Application	Title
human	IHC	Nature; Cell stress in cortical organoids impairs molecular subtype specification.; Authors - Aparna Bhaduri
human	WB	Nat Chem Biol; Targeting colorectal cancer with small-molecule inhibitors of ALDH1B1.; Authors - Zhiping Feng
human	IF	bioRxiv; Tropism of SARS-CoV-2 for Developing Human Cortical Astrocytes.; Authors - Madeline G Andrews
human	WB	Sci Adv; HPV is a cargo for the COPI sorting complex during virus entry; Authors - Mara C Harwood

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (05-14-2021)

very good for WB detection

• Applications: Western Blot

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