AQP6 Recombinant antibody

AQP6 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240175F9

Cat no : 83322-3-RR

Synonyms

Aquaporin6, Aquaporin 6, AQP 6, 240175F9

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Validation Data Gallery

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  WB analysis using 83322-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83322-3-RR (AQP6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83322-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83322-3-RR against Human AQP6 were performed. The affinity constant is 0.749 nM.

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Tested Applications

• Positive WB detected in: COLO 320 cells, HEK-293 cells, MCF-7 cells, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83322-3-RR is AQP6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: AQP6 fusion protein Ag26845
• Full Name: aquaporin 6, kidney specific
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: NM_001652
• Gene Symbol: AQP6
• Gene ID (NCBI): 363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AQP6 (Aquaporin-6) has recently been identified as an intracellular vesicle water channel with anion permeability that is activated by low pH or HgCl2 (PMID: 12034750). It could be involved in physiological mechanisms of fluid movement, acid-base regulation, and/or anion transport. The calculated MW of AQP6 is 29 kDa, but the actual observed MW is around 35 kDa, which represents glycosylated AQP6 monomer forms (PMID: 19811639).