AQP5 Polyclonal antibody, PBS Only

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  WB analysis using 20334-1-AP (same clone as 20334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20334-1-AP (AQP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 20334-1-AP (same clone as 20334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20334-1-AP (AQP5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

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  IF Staining of rat lung using 20334-1-AP (same clone as 20334-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat lung tissue using AQP5 antibody (20334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20334-1-PBS in a different storage buffer formulation.

AQP5 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 20334-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

aquaporin 5, Aquaporin-5, aquaporin5, AQP-5, AQP 5

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Product Information

20334-1-PBS targets AQP5 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AQP5 fusion protein Ag14103
• Full Name: aquaporin 5
• Calculated Molecular Weight: 265 aa, 28 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC032946
• Gene Symbol: AQP5
• Gene ID (NCBI): 362
• RRID: AB_2918066
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P55064
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

AQP5 is a member of aquaporins (AQPs) which are membrane proteins functioning as water channels and involved in the bidirectional transfer of water and small solutes across cell membranes. AQP5 is widely expressed including digestive, renal, respiratory, and reproductive systems. AQP5 overexpression in cancer cells and tumor tissues has been extensively reported. Recently AQP5 has been identified as a marker for adult pyloric stem cells.