Tested Applications
Positive WB detected in | 37°C incubated mouse brain tissue |
Positive IHC detected in | rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82296-6-RR targets Aquaporin 4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Aquaporin 4 fusion protein Ag9561 Predict reactive species |
Full Name | Aquaporin 4 |
Calculated Molecular Weight | 323 aa, 35 kDa |
Observed Molecular Weight | 32-34 kDa |
GenBank Accession Number | BC022286 |
Gene Symbol | Aquaporin 4 |
Gene ID (NCBI) | 361 |
RRID | AB_3670521 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | P55087 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.
Protocols
Product Specific Protocols | |
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WB protocol for Aquaporin 4 antibody 82296-6-RR | Download protocol |
IHC protocol for Aquaporin 4 antibody 82296-6-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |