Aquaporin 4 Recombinant antibody

• 

  WB analysis using 82296-6-RR

  37°C incubated mouse brain tissue were subjected to SDS PAGE followed by western blot with 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 82296-6-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 82296-6-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 82296-6-RR (Aquaporin 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 82296-6-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Aquaporin 4 antibody (82296-6-RR, Clone: 240856C7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 82296-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82296-6-RR against Human Aquaporin 4 were performed. The affinity constant is 5.06 nM.

Aquaporin 4 {ptg:Brand} Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 82296-6-RR

Clone No.240856C7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

AQP4, 240856C7, AQP 4, AQP-4, Aquaporin4

---

Tested Applications

• Positive WB detected in: 37°C incubated mouse brain tissue
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82296-6-RR targets Aquaporin 4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Aquaporin 4 fusion protein Ag9561
• Full Name: Aquaporin 4
• Calculated Molecular Weight: 323 aa, 35 kDa
• Observed Molecular Weight: 32-34 kDa
• GenBank Accession Number: BC022286
• Gene Symbol: Aquaporin 4
• Gene ID (NCBI): 361
• RRID: AB_3670521
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P55087
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aquaporins are specialized water transport channels in plasma membranes of water-permeable tissues. Aquaporin-4 (AQP4) is the most abundant water channel in the human central nervous system and is important to fluid movements in brain. Aquaporin-4 exists as two isoforms, a long (M1) isoform with translation initiation at Met-1, and a shorter (M23) isoform with translation initiation at Met-23, with molecular weights around 35-37 kDa and 32-34 kDa, respectively.