APPBP2 Polyclonal antibody

APPBP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12409-1-AP

Synonyms

APP BP2, APPBP2, HS.84084, KIAA0228, PAT1

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Validation Data Gallery

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  WB analysis using 12409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12409-1-AP (APPBP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12409-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12409-1-AP (APPBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Chromogenic substrates are DAB/H2O2 and CuSO4.

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12409-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12409-1-AP (APPBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A2780 cells, HeLa cells, Jurkat cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12409-1-AP targets APPBP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APPBP2 fusion protein Ag3092
• Full Name: amyloid beta precursor protein (cytoplasmic tail) binding protein 2
• Calculated Molecular Weight: 585 aa, 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC018121
• Gene Symbol: APPBP2
• Gene ID (NCBI): 10513
• RRID: AB_2918029
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amyloid proteinbinding protein 2 (APPBP2) is a hydrophilic tubulin that interacts with microtubules and is functionally involved in beta-amyloid precursor protein (APP) transport and/or processing. Based on the regulation of microtubules and APP, APPBP2 is found to be involved in the oncogenesis of various types of cancers, such as breast cancer, ovarian clear cell adenocarcinomas, desmoplastic medulloblastomas and neuroblastomas. Recently, Studies have demonstrated that APPBP2 is a potential target for diagnosis and therapeutic intervention in non-small cell lung cancer (NSCLC) (PMID: 31105033).

Publications

Species	Application	Title
mouse	WB	Diabetes; PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ; Authors - Yi Zhong