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APOL1 Recombinant antibody, PBS Only

APOL1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240814D11

Cat no : 83770-5-PBS

Synonyms

Apolipoprotein L-I, Apolipoprotein L I, ApoL-I, Apo-L, 240814D11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APOL1 Recombinant antibody (83770-5-RR)

    WB analysis using 83770-5-RR (same clone as 83770-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83770-5-RR (APOL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83770-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using APOL1 Recombinant antibody (83770-5-RR)

    IF Staining of HepG2 using 83770-5-RR (same clone as 83770-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using APOL1 antibody (83770-5-RR, Clone: 240814D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83770-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83770-5-RR

    Affinity and Kinetic Characterization of 83770-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83770-5-RR against Human APOL1 were performed. The affinity constant is below 1 pM.

Product Information

The immunogen of 83770-5-PBS is APOL1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen APOL1 fusion protein ag2016
Full Name apolipoprotein L, 1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC017331
Gene Symbol APOL1
Gene ID (NCBI) 8542
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles, acting as an interacting protein of apolipoprotein A1 (ApoA1). The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. APOL1 share structural and functional similarities with proteins of the Bcl-2 family and may has roles in apoptosis and autophagy. It is notable that APOL1 exists only in human and a few other primate species, and mouse does not express an APOL1 orthologue.