Apolipoprotein H Polyclonal antibody

Apolipoprotein H Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 11892-1-AP

Synonyms

APOH, B2G1, ApolipoproteinH, Apo-H, Apo H

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Validation Data Gallery

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  WB analysis using 11892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11892-1-AP (Apolipoprotein H antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 11892-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11892-1-AP (Apolipoprotein H antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human normal colon using 11892-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11892-1-AP (Apolipoprotein H antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 11892-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11892-1-AP (Apolipoprotein H antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11892-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Apolipoprotein H antibody (11892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse testis tissue
• Positive IHC detected in: human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

11892-1-AP targets Apolipoprotein H in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Apolipoprotein H fusion protein Ag2500
• Full Name: apolipoprotein H (beta-2-glycoprotein I)
• Calculated Molecular Weight: 345 aa, 38 kDa
• Observed Molecular Weight: 43-55 kDa
• GenBank Accession Number: BC026283
• Gene Symbol: APOH
• Gene ID (NCBI): 350
• RRID: AB_2058267
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Apolipoprotein H (ApoH), also known as beta2-glycoprotein I (B2-GPI), is a plasma glycoprotein, primarily synthesized in the liver. It has an important function in blood coagulation and clearance of apoptotic bodies from the circulation. ApoH is also an important actor of host innate immune response through its capacity to bind with high affinity to a large panel of pathogens or their proteins. ApoH is a 54 kDa plasma glycoprotein.

Publications

Species	Application	Title
human	IHC	Nat Commun; ATRX, DAXX or MEN1 mutant pancreatic neuroendocrine tumors are a distinct alpha-cell signature subgroup.; Authors - Chang S Chan
human	IP	Neuropharmacology; Anti-β2GPI/β2GPI induces neutrophil extracellular traps formation to promote thrombogenesis via the TLR4/MyD88/MAPKs axis activation.; Authors - Caijun Zha
human,mouse	WB	J Biol Chem; Development of betabodies: the next generation of phosphatidylserine targeting agents; Authors - Natalie Z Phinney
human	WB	Cancer Med; Identification of novel pQTL-SNPs associated with lung adenocarcinoma risk: A multi-stage study; Authors - Yutong Wu