APOBEC3G Polyclonal antibody

APOBEC3G Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10608-1-AP

Synonyms

A3G, APOBEC related protein, APOBEC related protein 9, APOBEC3G, ARCD, ARP 9, ARP9, bK150C2.7, CEM 15, CEM15, MDS019

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Validation Data Gallery

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  WB analysis of mouse colon using 10608-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 10608-1-AP (APOBEC3G Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 10608-1-AP

  IP result of anti-APOBEC3G (IP:10608-1-AP, 4ug; Detection:10608-1-AP 1:300) with COLO 320 cells lysate 2240ug.

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  IHC staining of human testis using 10608-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 10608-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 10608-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10608-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10608-1-AP (APOBEC3G Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of MCF-7 using 10608-1-AP

  Immunofluorescent analysis of MCF-7 cells using 10608-1-AP (APOBEC3G antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse colon tissue
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human testis tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

10608-1-AP targets APOBEC3G in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APOBEC3G fusion protein Ag0887
• Full Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC024268
• Gene Symbol: APOBEC3G
• Gene ID (NCBI): 60489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APOBEC3G((apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G,also known as CEM15), a member of the APOBEC family of cytidine deaminase-editing enzymes, is a potent DNA mutator that can be packaged into budding retroviruses.HA-tagged APOBEC3G can be expressed in 293T cells, which do not express APOBEC3G endogenously,in addition 22- and 24-kDa proteolytic degradation fragments can be detected by western blot(PMID:14672928). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Nat Immunol; Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.; Authors - Li Jianhua J
human	WB,IHC	J Clin Invest; APOBEC3G promotes liver metastasis in an orthotopic mouse model of colorectal cancer and predicts human hepatic metastasis.; Authors - Ding Qingqing Q
human	WB	Cancers (Basel); Overexpression of NNMT in Glioma Aggravates Tumor Cell Progression: An Emerging Therapeutic Target; Authors - Wei Sun