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APOBEC3A Recombinant antibody, PBS Only (Capture)

APOBEC3A Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241529F1

Cat no : 84239-2-PBS

Synonyms

PHO1, EC:3.5.4.38, DNA dC->dU-editing enzyme APOBEC-3A, ARP3, A3A

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using APOBEC3A Recombinant antibody (84239-2-RR)

    IF Staining of THP-1 using 84239-2-RR (same clone as 84239-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using APOBEC3A antibody (84239-2-RR, Clone: 241529F1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84239-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01122-3

    Cytometric bead array standard curve of MP01122-3

    Cytometric bead array standard curve of MP01122-3, APOBEC3A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84239-2-PBS. Detection antibody: 84239-3-PBS. Standard: Ag18845. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84239-2-RR

    Affinity and Kinetic Characterization of 84239-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84239-2-RR against Human APOBEC3A were performed. The affinity constant is below 1 pM.

Product Information

84239-2-PBS targets APOBEC3A as part of a matched antibody pair:

MP01122-3: 84239-2-PBS capture and 84239-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen APOBEC3A fusion protein Ag18845
Full Name apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3A
Calculated Molecular Weight 199 aa, 23 kDa
GenBank Accession NumberBC126416
Gene Symbol APOBEC3A
Gene ID (NCBI) 200315
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

APOBEC3A, also known as A3A, belongs to the cytidine and deoxycytidylate deaminase family. APOBEC3A plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. APOBEC3A is expressed in several cell types, including keratinocytes and myeloid cells (PMID: 28825669). APOBEC3A has 2 isoforms with the molecular mass of 22 and 23 kDa.