APOBEC2 Polyclonal antibody, PBS Only

APOBEC2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20121-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 20121-1-AP (same clone as 20121-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20121-1-AP (APOBEC2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 20121-1-AP (same clone as 20121-1-PBS)

  IP result of anti-APOBEC2 (IP:20121-1-AP, 4ug; Detection:20121-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug. This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 20121-1-AP (same clone as 20121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20121-1-AP (APOBEC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 20121-1-AP (same clone as 20121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20121-1-AP (APOBEC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 20121-1-AP (same clone as 20121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20121-1-AP (APOBEC2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 20121-1-AP (same clone as 20121-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 20121-1-AP (APOBEC2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20121-1-PBS in a different storage buffer formulation.

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Product Information

20121-1-PBS targets APOBEC2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APOBEC2 fusion protein Ag13699
• Full Name: apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 2
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 25-30 kDa
• GenBank Accession Number: BC047767
• Gene Symbol: APOBEC2
• Gene ID (NCBI): 10930
• RRID: AB_10697687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

APOBEC2, also named as ARCD1 and ARP1, is a member of the activation-induced deaminase/apolipoprotein B mRNA editing enzyme catalytic polypeptide (APOBEC) cytidine deaminase family expressed in differentiated skeletal and cardiac muscle. APOBEC family members have diverse roles by their ability to edit DNA and/or RNA. Apobec2 is one of the oldest members of the APOBEC family, along with the lymphoid-specific activation-induced deaminase. Unlike other APOBEC family members, the enzymatic activity and substrate of Apobec2 have not been fully demonstrated, and its biologic functions remain unknown. It can be detected as 25-30 kDa.