APOB Recombinant antibody, PBS Only

APOB Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

240884H4

Cat no : 84047-1-PBS

Synonyms

Apolipoprotein B, ApoB48, ApoB100, Apo B-100, Apo B

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Validation Data Gallery

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  WB analysis of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 84047-1-RR (APOB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84047-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84047-1-RR (APOB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84047-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using APOB antibody (84047-1-RR, Clone: 240884H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84047-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 84047-1-RR (same clone as 84047-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using APOB antibody (84047-1-RR, Clone: 240884H4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84047-1-PBS in a different storage buffer formulation.

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Product Information

84047-1-PBS targets APOB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: apolipoprotein B (including Ag(x) antigen)
• Calculated Molecular Weight: 516 kDa
• Observed Molecular Weight: 150-250 kDa, 400-520 kDa
• GenBank Accession Number: NM_000384
• Gene Symbol: APOB
• Gene ID (NCBI): 338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 (PMID:11839763, PMID:2450346).