APLP2 Polyclonal antibody

APLP2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA, PLA

Conjugate

Unconjugated

Publications

3

Cat no : 15041-1-AP

Synonyms

APLP-2, Amyloid-like protein 2, Amyloid like protein 2, Amyloid beta precursor like protein 2, Amyloid beta

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Validation Data Gallery

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  WB analysis using 15041-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15041-1-AP (APLP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15041-1-AP

  IP result of anti-APLP2 (IP:15041-1-AP, 4ug; Detection:15041-1-AP 1:1000) with mouse brain tissue lysate 2100 ug.

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  IHC staining of mouse brain using 15041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15041-1-AP (APLP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15041-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15041-1-AP (APLP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

15041-1-AP targets APLP2 in WB, IHC, IP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APLP2 fusion protein Ag6838
• Full Name: amyloid beta (A4) precursor-like protein 2
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC000373
• Gene Symbol: APLP2
• Gene ID (NCBI): 334
• RRID: AB_2289597
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Amyloid precursor-like protein-2 (APLP2) is a member of the APP (amyloid precursor protein) family including APP, APLP1, and APLP2. APLP2 is ubiquitously expressed. It contains heparin-, copper- and zinc- binding domains at the N-terminus, BPTI/Kunitz inhibitor and E2 domains in the middle region, and transmembrane and intracellular domains at the C-terminus. APLP2 has been shown to regulate multiple cellular functions such as neurite outgrowth, axogenesis, corneal epithelial wound healing, cell adhesion, migration, and mitosis. The synergy of this protein and the APP is required to mediate neuromuscular transmission, spatial learning and synaptic plasticity. APLP2 has been implicated in the pathogenesis of Alzheimer's disease.

Publications

Species	Application	Title
		iScience; Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia; Authors - Yu-Han H Hsu
human	WB,PLA	Life Sci Alliance; Quantitative proteomics identifies PTP1B as modulator of B cell antigen receptor signaling.; Authors - Jennifer J Schwarz
human,mouse	WB	Heliyon; HMGA2 overexpression activates IGF2BP2 to stabilize APLP2 via m6A modification and promote pancreatic cancer progression; Authors - Ke Liu