APLF Polyclonal antibody

APLF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14252-1-AP

Synonyms

APFL, APLF, Aprataxin and PNK like factor, aprataxin and PNKP like factor, C2orf13, PALF, Xip1, XRCC1 interacting protein 1

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Validation Data Gallery

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  WB analysis of PC-3 using 14252-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14252-1-AP (APLF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14252-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14252-1-AP (APLF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14252-1-AP targets APLF in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APLF fusion protein Ag5525
• Full Name: aprataxin and PNKP like factor
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC041144
• Gene Symbol: APLF
• Gene ID (NCBI): 200558
• RRID: AB_10641990
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Nat Struct Mol Biol; Mechanism for recycling tRNAs on stalled ribosomes.; Authors - Matthew C J Yip