Various lysates were subjected to SDS PAGE followed by western blot with 67781-1-Ig (APEX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67781-1-Ig (APEX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
WB analysis of HeLa using 67781-1-Ig
WB result of APEX1 antibody (67781-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APEX1 transfected HeLa cells.
WB result of APEX1 antibody (67781-1-Ig; 1:15000; room temperature for 1.5 hours) with wild-type and APEX1 knockout HuH-7 cells.
WB analysis using 67781-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67781-1-Ig (APEX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67781-1-Ig (APEX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of mouse liver using 67781-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67781-1-Ig (APEX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
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mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
67781-1-Ig targets APEX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression.
Various lysates were subjected to SDS PAGE followed by western blot with 67781-1-Ig (APEX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
WB analysis of HeLa using 67781-1-Ig
WB result of APEX1 antibody (67781-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APEX1 transfected HeLa cells.
WB analysis of HuH-7 using 67781-1-Ig
WB result of APEX1 antibody (67781-1-Ig; 1:15000; room temperature for 1.5 hours) with wild-type and APEX1 knockout HuH-7 cells.
WB analysis using 67781-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67781-1-Ig (APEX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse liver using 67781-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67781-1-Ig (APEX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 67781-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67781-1-Ig (APEX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
APEX1 Monoclonal antibody
Catalog Number
67781-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:1000-1:4000
Applications
WB, IHC, ELISA
Reactivity
human, mouse, rat, pig, rabbit
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
