APEX1 Polyclonal antibody

APEX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10323-1-AP

Synonyms

APEX nuclease, APEX, APEN, APE-1, APE1

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Validation Data Gallery

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  WB analysis of HeLa using 10323-1-AP

  WB result of APEX1 antibody (10323-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-APEX1 transfected HeLa cells.

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  WB analysis of HepG2 using 10323-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10323-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10323-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10323-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10323-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10323-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10323-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 10323-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 10323-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10323-1-AP (APEX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 10323-1-AP

  IP result of anti-APEX1 (IP:10323-1-AP, 4ug; Detection:10323-1-AP 1:500) with HepG2 cells lysate 2200 ug.

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  IHC staining of human cervical cancer using 10323-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 10323-1-AP (APEX1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 10323-1-AP

  Immunofluorescent analysis of HepG2 cells using 10323-1-AP (APEX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, HeLa cells, K-562 cells, mouse liver tissue, Raji cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10323-1-AP targets APEX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APEX1 fusion protein Ag0397
• Full Name: APEX nuclease (multifunctional DNA repair enzyme) 1
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC004979
• Gene Symbol: APEX1
• Gene ID (NCBI): 328
• RRID: AB_2057925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APEX1, also named as APE, APE1, HAP1 and REF-1, belongs to the DNA repair enzymes AP/ExoA family. It is a multifunctional protein that plays a central role in the cellular response to oxidative stress. The two major activities of APEX1 are in DNA repair and redox regulation of transcriptional factors. APEX nuclease is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime,5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. On the other hand, APEX1 also exerts reversible nuclear redox activity to regulate DNA binding affinity and transcriptional activity of transcriptional factors by controlling the redox status of their DNA-binding domain, such as the FOS/JUN AP-1 complex after exposure to IR. APEX1 is involved in calcium-dependent down-regulation of parathyroid hormone (PTH) expression by binding to negative calcium response elements (nCaREs). When acetylated at Lys-6 and Lys-7, APEX1 stimulates the YBX1-mediated MDR1 promoter activity, leading to drug resistance. It also acts as an endoribonuclease involved in the control of single-stranded RNA metabolism. It plays a role in regulating MYC mRNA turnover by preferentially cleaving in between UA and CA dinucleotides of the MYC coding region determinant (CRD). In association with NMD1, APEX1 plays a role in the rRNA quality control process during cell cycle progression. 10323-1-AP is a rabbit polyclonal antibody raised against a fusion protein corresponding to an internal region of human APEX1.