AARE Polyclonal antibody

AARE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14758-1-AP

Synonyms

APEH, Acyl-peptide hydrolase, Acylaminoacyl-peptidase, Acylaminoacyl peptidase, Acylamino-acid-releasing enzyme

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Validation Data Gallery

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  WB analysis using 14758-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14758-1-AP (AARE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 14758-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14758-1-AP (AARE antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 14758-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14758-1-AP (AARE antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14758-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AARE antibody (14758-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, human plasma tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

14758-1-AP targets AARE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AARE fusion protein Ag6511
• Full Name: N-acylaminoacyl-peptide hydrolase
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 66-80 kDa
• GenBank Accession Number: BC000362
• Gene Symbol: APEH
• Gene ID (NCBI): 327
• RRID: AB_2057811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APH, (APEH, acylpeptide hydrolase), which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. A range of molecular masses have been reported for APH by previous investigators, including 80 kD, 75 kD and 69 kD. This antibody can recognize at least 2 isoforms of APH.

Publications

Species	Application	Title
	WB	mSphere; Internalization of Erythrocyte Acylpeptide Hydrolase Is Required for Asexual Replication of Plasmodium falciparum.; Authors - Rubayet Elahi