APCDD1 Recombinant antibody

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  WB analysis using 83246-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83246-6-RR (APCDD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 83246-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83246-6-RR (APCDD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83246-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83246-6-RR against Human APCDD1 were performed. The affinity constant is 0.634 nM.

APCDD1 {ptg:Brand} Recombinant Antibody for WB, ELISA

Cat No. 83246-6-RR

Clone No.240085C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Protein APCDD1, FP7019, DRAPC1, B7323, Adenomatosis polyposis coli down-regulated 1 protein

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Tested Applications

• Positive WB detected in: SKOV-3 cells, Raji cells, mouse heart tissue, HEK-293T cells, HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83246-6-RR targets APCDD1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: APCDD1 fusion protein Ag10303
• Full Name: adenomatosis polyposis coli down-regulated 1
• Calculated Molecular Weight: 514 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC053324
• Gene Symbol: APCDD1
• Gene ID (NCBI): 147495
• RRID: AB_3670921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8J025
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APCDD1(adenomatosis polyposis coli down-regulated 1) is a membrane-bound glycoprotein conserved during vertebrate evolution that regulates important biological processes controlled by Wnt signaling (PMID: 26170146). APCDD1 is a membrane-bound glycoprotein that is abundantly expressed in human HFs (hair follicles) and can interact in vitro with WNT3A and LRP5, two essential components of Wnt signaling (PMID: 20393562). APCDD1 may be modified by glycosylation.