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APBB1 Polyclonal antibody, PBS Only

APBB1 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27874-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using APBB1 Polyclonal antibody (27874-1-AP)

    WB analysis using 27874-1-AP (same clone as 27874-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27874-1-AP (APBB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27874-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using APBB1 Polyclonal antibody (27874-1-AP)

    WB analysis using 27874-1-AP (same clone as 27874-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27874-1-AP (APBB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27874-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using APBB1 Polyclonal antibody (27874-1-AP)

    IP experiment of mouse brain using 27874-1-AP (same clone as 27874-1-PBS)

    IP result of anti-APBB1 (IP:27874-1-AP, 4ug; Detection:27874-1-AP 1:3000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 27874-1-PBS in a different storage buffer formulation.

Product Information

27874-1-PBS targets APBB1 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APBB1 fusion protein Ag27434
Full Name amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Calculated Molecular Weight 708 aa, 77 kDa
Observed Molecular Weight 65 kDa, 97 kDa
GenBank Accession NumberBC010854
Gene Symbol APBB1
Gene ID (NCBI) 322
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Adaptor protein FE65 (APBB1) specifically binds to the intracellular tail of the type I transmembrane protein, β-amyloid precursor protein (APP). FE65 is a multimodular adaptor protein, consisting of three major protein-protein interaction domains, a WW domain and two phosphotyrosine interaction domains (PID). The interaction between FE65 and APP mainly takes place between the C-terminal PID (PID2) and the APP intracellular domain (AICD) (PMID: 15647266). There is novel evidence that the major isoform of FE65 (97-kDa FE65, p97FE65) can be converted to a 65-kDa N-terminally truncated C-terminal fragment (p65FE65) via endoproteolysis. The cleavage region locates immediately after an acidic residue cluster but before the three major protein-protein binding domains (PMID: 15647266). FE65 is predominantly expressed in central nervous system neurons, and its expression is regulated during development and aging, with high levels corresponding to the timing of neural tissue formation and high neuronal activity (PMID: 7867517, PMID: 10561691, PMID: 14689444). p65FE65 (65kDa) is endoproteolytically cleaved from p97FE65 (97 kDa), the major isoform of FE65 (97-kDa FE65, p97FE65) can be converted to a 65-kDa N-terminally truncated C-terminal fragment (p65FE65) via endoproteolysis (PMID: 15647266).