APBA1 Polyclonal antibody

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  WB analysis using 55089-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55089-1-AP (APBA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 55089-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55089-1-AP (APBA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 55089-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55089-1-AP (APBA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

APBA1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55089-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

X11, Neuron specific X11 protein, MINT1, Mint 1, D9S411E

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Tested Applications

• Positive WB detected in: Neuro-2a cells, SH-SY5Y cells, SK-N-SH cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55089-1-AP targets APBA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: amyloid beta (A4) precursor protein-binding, family A, member 1
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 138-140 kDa
• GenBank Accession Number: NM_001163
• Gene Symbol: APBA1
• Gene ID (NCBI): 320
• RRID: AB_3670237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02410
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APBA1, also named MINT1 and X11A, is a brain-specific multi-modular protein that interacts with the Alzheimer's disease amyloid precursor protein (APP)(PMID: 22136355). APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. APBA1 is expressed in the brain and was found to interact with Munc18.