APAF1 Polyclonal antibody, PBS Only

• 

  WB analysis of COLO 320 using 29022-1-AP (same clone as 29022-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 29022-1-AP (APAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29022-1-PBS in a different storage buffer formulation.

APAF1 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 29022-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

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Product Information

29022-1-PBS targets APAF1 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APAF1 fusion protein Ag30606
• Full Name: apoptotic peptidase activating factor 1
• Calculated Molecular Weight: 1248 aa, 142 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC136532
• Gene Symbol: APAF1
• Gene ID (NCBI): 317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O14727
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.