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APAF1 Polyclonal antibody, PBS Only

APAF1 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29022-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using APAF1 Polyclonal antibody (29022-1-AP)

    WB analysis of COLO 320 using 29022-1-AP (same clone as 29022-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 29022-1-AP (APAF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29022-1-PBS in a different storage buffer formulation.

Product Information

29022-1-PBS targets APAF1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen APAF1 fusion protein Ag30606
Full Name apoptotic peptidase activating factor 1
Calculated Molecular Weight 1248 aa, 142 kDa
Observed Molecular Weight130-140 kDa
GenBank Accession NumberBC136532
Gene Symbol APAF1
Gene ID (NCBI) 317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.