AP50 Polyclonal antibody

• Featured Product
• KD/KO Validated

AP50 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 27355-1-AP

Synonyms

Adaptin mu2, AP 2 complex subunit mu, AP 2 mu chain, AP2M1, AP50, CLAPM1, HA2 50 kDa subunit, KIAA0109, mu2

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Validation Data Gallery

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  WB analysis using 27355-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27355-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 27355-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 27355-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27355-1-AP (AP50 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 27355-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27355-1-AP (AP50 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 27355-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27355-1-AP (AP50 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells, rat brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

The immunogen of 27355-1-AP is AP50 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AP50 fusion protein Ag26059
• Full Name: adaptor-related protein complex 2, mu 1 subunit
• Calculated Molecular Weight: 433 aa, 49 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC013796
• Gene Symbol: AP50
• Gene ID (NCBI): 1173
• RRID: AB_2880853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF,IP	Autophagy; AP2M1 mediates autophagy-induced CLDN2 (claudin 2) degradation through endocytosis and interaction with LC3 and reduces intestinal epithelial tight junction permeability.; Authors - Ashwinkumar Subramenium Ganapathy ; KO Validated
human,mouse	WB	Cell Commun Signal; SUMOylation-induced membrane localization of TRPV1 suppresses proliferation and migration in gastric cancer cells; Authors - Yang Yang
human	IHC	Oral Dis; Expression levels of SIX1, ME2, and AP2M1 in adenoid cystic carcinoma and mucoepidermoid carcinoma.; Authors - Cong-Cong Wu
human	IHC	Cancer Res; Lipid metabolic reprogramming extends beyond histological tumor demarcations in operable human pancreatic cancer; Authors - Juho Pirhonen
mouse	WB	Nat Commun; Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis; Authors - Yesheng Fu
human	IHC	J Transl Med; A multi-dimensional approach to unravel the intricacies of lactylation related signature for prognostic and therapeutic insight in colorectal cancer; Authors - Huixia Huang